SOPs

VITAL SOPs Please note that this is not the full set of SOPs - we are still awaiting some of them. If you notice a mistake in any of these SOPs, please contact Wim or David Rodriguez

	001. Sampling and virus concentration from faeces	002. Sampling and virus concentration from harvesters' hands	003. Sampling and virus concentration from animal-derived fertilizer

004. Sampling and virus concentration from waters	005. Sampling and virus concentration from soft fruit	006. Sampling and virus concentration from vegetables	007. Sampling and virus concentration from shellfish

008. Sampling and virus concentration from blood	009. Sampling and virus concentration from pork meat and liver tissue	010. Nucleic acids extraction from faeces, animal-derived fertilizer, or blood	011. Nucleic acids extraction from pork liver tissue or meat

012. Nucleic acids extraction from soft fruits, vegetables or shellfish	013. Nucleic acids extraction from irrigation water, slaughterhouse effluents, or harvesters' hands wash-off	014. General adenovirus QPCR	015. Detection and quantification of porcine adenoviruses by real-time PCR

016. Detection and quantification of Bovine polyomavirus by real-time PCR	017. Human adenovirus nested PCR	018. Detection and quantification of Norovirus by real-time reverse transcriptase PCR	019. Detection and quantification of hepatitis A virus by real-time reverse transcriptasePCR

020. Detection and Quantification of hepatitis E virus by real-time reverse transcriptase PCR	021. Detection and quantification of murine norovirusby real-time reverse transcriptase PCR	022. Virus detection by (RT-)PCR: details on quality controls, virus detection and quantification	023. Protocol for the establishment of IAC incorporation, optimised for each laboratory

Spiking of raspberries with 5 x 10ulsuspension